Antioxidant Properties of Marrubium peregrinum L. (Lamiaceae) Essential Oil
نویسندگان
چکیده
The antioxidant activity of Marrubium peregrinum essential oil, collected from three different locations [Backo Gradiste Rimski Sanac (No.1), Novi Knezevac (No.2) and Senta (No.3)] was evaluated as free radical scavenging capacity (RSC), together with inhibition on xanthine-oxidase and effects on lipid peroxidation (LP). RSC was assessed measuring the scavenging activity of the essential oils on 2,2-diphenyl-1-picrylhydrazyl (DPPH), super oxide anion (O2), nitric-oxide (NO) and hydroxyl (OH) radicals. The activities of xanthine-oxidase (XOD) was determined by the nitrite method. Effects on LP were evaluated by following the activities of essential oils in the Fe/ascorbate induction system. Experimental results indicate that the essential oil of M. peregrinum from Senta (No.3) exhibited the strongest inhibitory effect, as the IC50 value was achieved with the lowest concentration. The same result was obtained in investigation of influence of essential oil on XOD and LP. The chemical profile of essential oil was evaluated by the means of gas chromatography-mass spectrometry (GC-MS). According to the analysis, the most powerful scavenging compounds were sesquiterpene hydrocarbons (-caryophyllene, bicyclogermacrene and germacrene-D) and oxygenated sesquiterpenes (spathulenol and caryophyllene oxide). OPEN ACCESS Molecules 2010, 15 5944
منابع مشابه
TOTAL PHENOLIC CONTENT, FLAVONOID CONCENTRATION AND ANTIOXIDANT ACTIVITY OF Marrubium peregrinum L. EXTRACTS
In this study, in vitro antioxidant activity, total phenolic content and concentration of flavonoids of five different extracts, from the whole herb of Marrubium peregrinum L. (Lamiaceae) were determined using spectrophotometric methods. Antioxidant activity of extracts were expressed as percentage of DPPH radicals inhibition and IC50 values (μg/ml). Values in percentage ranged from 27.26 to 89...
متن کاملEXPLORING ANTIMICROBIAL ACTIVITY OF HOREHOUND, Marrubium peregrinum L. EXTRACTS
Antimicrobial activity of methanol, acetone and ethyl acetate extracts from whole herb of Marrubium peregrinum L. (Lamiaceae) were investigated in this study. Horehound, a perennial plant, was collected from the region of Suva Planina Mt. in eastern Serbia. Testing was preformed by microdilution method and minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) have ...
متن کاملAntibiofilm formation, antioxidant and anticholinesterase activities of essential oil and methanol extract of Marrubium deserti de Noé
The essential oil obtained from the aerial parts of Marrubium deserti de Noé. (Lamiaceae), growing in the North fringe of the Algerian Sahara, was analyzed by GC-MS. Thirty-eight compounds were identified, representing 99.70% of the total oils. The GC-MS analysis revealed the presence of tetracosane, germacrene D, Δ-cadinene, α-cadinol and t-cadinol as the main constituents, representing 31.11%...
متن کاملCharacterization of Non-Terpenoids in Marrubium crassidens Boiss. Essential Oil.
PURPOSE Marrubium crassidens, a plant belonging to the family Lamiaceae, was studied for its volatile components present in the aerial parts of the plant during the flowering stage. METHODS The essential oil of the plant obtained through hydrodistillation of the dried plant material was assessed for its chemical composition by GC/MS and GC-FID analyses. RESULTS Twenty-five compounds were id...
متن کاملA study on some secondary metabolites and quantity and quality of essential oil of Marrubium vulgare L. grown in Guynik district (North Khorasan province)
To evaluate the biological potential and medicinal properties, the biochemical compositions and quantity and quality of essential oil of Marrubium vulgare L., grown in the natural habitat located in Guynik region, North Khorasan province, were investigated. The content of secondary metabolites including phenol and flavonoids, and antioxidant capacity were measured in laboratory methods. Essenti...
متن کامل